Department of Microbiology
Akio Nomoto, Ph.D.
Tetsuro Matano, M.D., Ph.D.
Takeshi Yamada, Ph.D., Seii Ohka, Ph.D.
Microbial disease has been recognized as the major threat to human health
throughout the history. Despite the development of preventive and therapeutic
interventions against some pathogenic microbes, infectious disease is still one
of the most significant medical problems. On the other hand, microbial organisms
have served as a useful model as well for elucidating the molecular mechanisms
of a variety of biological events, providing useful insights into life science.
Recently, efforts have also been initiated by a number of research groups to
utilize animal viruses as a tool for human gene therapy. In order to familiarize
students with these issues, importance of microbiology in medical education is
increasing more rapidly than ever. To fulfill this requirement, our department,
as the only basic microbiology unit in the Faculty, currently assumes a
responsibility for teaching bacteriology, mycology, and virology to medical
Undergraduate Course, Faculty of Medicine
In a series of lectures (totally 60 hr) and laboratory courses (30 hr), the
following subjects are covered.
1) Molecular biology of bacteria, phages, and animal viruses
2) Mechanisms of microbial diseases
3) Laboratory diagnosis of pathogenic microbes
4) Infection control and biosafety
5) Application of microbial organisms for biotechnology
6) Socioeconomic impact of microbial diseases
In addition to the staff of our department, experts from the National Institutes
of Infectious Diseases (Dr. Shimada), Dokkyo Medical University (Dr. Masuda),
Teikyo University (Dr. Kawana), Faculty of Agriculture (Dr. Onodera), and
Institute of Medical Science (Dr. Sasakawa, Dr. Kawaoka, Dr. Iwamoto, and Dr.
Saito) contribute to the teaching activities.
1. IRES (internal ribosome entry site)-dependent virus tropism
Honaga, T., Ohka, S., and Nomoto, A.
2. Poliovirus entry into cells and transport
Sakai, M., Ohka, S., and Nomoto, A.
3. Natural function of poliovirus receptor
Oda, T., Ohka, S., and Nomoto, A.
4. Intracellular immunity of neural cells against poliovirus infection
Mitusda, J., Matsuda, N., Hosoda, N., Ohka, S., and Nomoto, A.
5. Translational control - ribosome recycling-
Hosoda, N., Ohka, S., and Nomoto, A.
6. Poliovirus replication and development of poliovirus vector
Yoshino, Y., Fujimaki, T., Ohka, S., and Nomot, A.
7. HCV (hepatitis C virus) genome as an RNA replicon
Yamada, T., Shigenobu, F., Arimoto, K., and Nomoto, A.
8. Molecular basis for HCV pathogenesis
Yamada, T., Murayama, A., and Nomoto, A.
9. Molecular basis for retroviral pathogenesis
Kusuki, K., Sasaki, Y., Kizaki, M., Takeda, A., Igarashi, H., and Matano, T.
10. Virus-specific immune responses
Kawada, M., Kobayashi, M., Tsukamoto, T., Takeda, A., Igarashi, H., and Matano,
11. Development of AIDS vaccine
Yamamoto, H., Kato, M., Lun, W.H., Kano, M., Takeda, A., Igarashi, H., and
- Jia Q, Liang F, Ohka S, Nomoto A, Hashikawa T. Expression of
brain-derived neurotrophic factor in the central nervous system of mice
using a poliovirus-based vector. J NeuroVirology 8(1): 14-23, 2002.
- Nomoto A, Arita I. Eradication of Poliomyelitis. Nature Immunology 3(3):
- Feng JY, Ishibashi T, Nomoto A, Masuda M. Isolation and analysis of
retroviral integration targets by solo long terminal repeat inverse PCR. J
Virol 76(11): 5540-5547, 2002.
- Gromeier M, Nomoto A. Determinants of poliovirus pathogenesis. In
Molecular Biology of Picornaviruses, Bert L. Semler and Eckard Wimmer eds.
ASM Press, pp367-379, 2002.
- Iwasaki A, Welker R, Mueller S, Linehan M, Nomoto A, Wimmer E.
Immunofluorescence analysis of poliovirus receptor expression in Peyer's
patches of humans, primates, and CD155 transgenic mice: implication for
poliovirus infection. J Inf Dis 186(5): 585-592, 2002.
- Masuda M, Yageta M, Fukuhara H, Kuramochi M, Maruyama T, Nomoto A,
Murakami Y. The tumor suppressor protein TSLC1 is involved in cell-cell
adhesion. J Biol Chem 277(34): 31014-31019, 2002.
- Isoyama T, Kuge S, Nomoto A. The core protein of hepatitis C virus is
imported into the nucleus by transport receptor Kap123p but inhibits
Kap121p-dependent nuclear import of yeast-AP1-like factor in yeast cells. J
Biol Chem 277(42): 39634-39641, 2002.
- Horie H, Yoshida H, Matsuura K, Miyazawa M, Wakabayashi K, Nomoto A,
Hashizume S. Isolation of vaccine-derived type 1 polioviruses displaying
similar properties to virulent wild strain Mahoney from sewage in Japan. J
Med Virol 68: 445-451, 2002.
- Kano M, Matano T, Kato A, Nakamura H, Takeda A, Suzaki Y, Ami Y, Terao
K, Nagai Y. Primary replication of a recombinant Sendai viral vector in
macaques. J Gen Virol 83: 1377-1386, 2002.
- Kano M, Matano T, Kato A, Shioda T, Nagai Y. Induction of HIV-1-specific
neutralizing antibodies in mice vaccinated with a recombinant Sendai virus
vector. Jpn J Infect Dis 55: 59-60, 2002.
- Matano T. Recent advances in AIDS vaccine preclinical trials: challenges
against the chronic disease. Current Topics in Virology 2: 179-185, 2002.
- Pelletier I, Ouzilou L, Arita M, Nomoto A, Colbere-Garapin F.
Characterization of the poliovirus 147S particles: new insights into
poliovirus uncoating. Virology 305(1): 55-65, 2003.
- Ida-Hosonuma M, Sasaki Y, Toyoda H, Nomoto A, Gotoh O, Yonekawa H, Koike
S. Host range of poliovirus is restricted to simians because of a rapid
sequence change of the poliovirus receptor gene during evolution. Arch Virol
- Yanagiya A, Ohka S, Hashida N, Okamura M, Taya C, Kamoshita N, Iwasaki
K, Sasaki Y, Yonekawa H, Nomoto A. Tissue specific replicating capacity of a
chimeric poliovirus that carries the internal ribosome entry site of
hepatitis C virus in a new mouse model transgenic for human poliovirus
receptor. J Virol 77(19): 10479-10487, 2003.
- Matano T, Kano M, Takeda A, Nakamura H, Nomura N, Furuta Y, Shioda T,
Nagai Y. No significant enhancement of protection by Tat-expressing Sendai
viral vector-booster in a macaque AIDS model. AIDS 17: 1392-1394, 2003.
- Takeda A, Nakamura H, Matano T. Evaluation of simian immunodeficiency
virus-specific immune responses induced by a defective proviral DNA vaccine
in macaques. Jpn J Infect Dis 56: 172-173, 2003.
- Takeda A, Igarashi H, Nakamura H, Kano M, Iida A, Hirata T, Hasegawa M,
Nagai Y, Matano T. Protective efficacy of an AIDS vaccine, a single
DNA-prime followed by a single booster with a recombinant
replication-defective Sendai virus vector, in a macaque AIDS model. J Virol
77: 9710-9715, 2003.
- Fujiyuki T, Takeuchi H, Ono M, Ohka S, Sasaki T, Nomoto A, Kubo T. A
novel insect picorna-like virus identified in the brain of aggressive worker
honeybees. J Virol 78(3): 1093-1100, 2004.
- Nagata N, Iwasaki T, Ami Y, Sato Y, Hatano I, Harashima A, Suzaki Y,
Yoshii T, Hashikawa T, Sata T, Horiuchi Y, Koike S, Kurata T, Nomoto A. A
poliomyelitis model through mucosal infection in transgenic mice bearing
human poliovirus receptor, TgPVR21. Virology, in press.
- Ohka S, Matsuda N, Tohyama K, Oda T, Morikawa M, Kuge S, Nomoto A.
Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal
transport of the endosome. J Virol, in press.
- Hanaki K, Ohka S, Yamamoto K, Nomoto A, Yoshikura H. Oligo(dA-dT)
dependent signal amplification for the detection of proteins in cells. Bio
Techniques, in press.
Annual Report of the Graduate School of Medicine and The Faculty of Medicine
The University of Tokyo Reports for the Period April 2002 - March 2004